Nephroprotective potential of Trichosanthes dioica ROXB. leaves extract against Gentamicin induced Nephropathy in albino Rats

Abstract

The purpose of this study is to evaluate nephroprotective potential of Trichosanthes dioica leaves extract against Gentamicin (GM) induced nephrotoxicity and renal dysfunction. Twenty-four Wistar rats were divided into four groups (n = 6). Control rats that received normal saline (i.p.) and 0.5% carboxymethyl cellulose (p.o.) per day for 8 days. Nephrotoxicity was induced in rats by intraperitoneally 
administration of GM (100 mg/kg/day for 8 days) and were treated with TLE (200 and 400 mg/kg/day (p.o.) for 8 days). Plasma and urine urea and creatinine, kidney weight, urine output, blood urea nitrogen, urinary sodium and potassium level, renal enzymatic and non-enzymatic antioxidants and lipid peroxidation was evaluated along with histopathological investigation in various experimental groups of rats. It was observed that the GM treatment induced significant elevation (p < 0.001) in plasma and urine urea, creatinine, kidney weight, + + blood urea nitrogen, plasma Na and K level, renal lipid peroxidation along with significant decrement (p< 0.001) in 
urine output, renal enzymatic and non-enzymatic antioxidants. TLE 200 and 400 mg/kg treatment to GM treated rats recorded significant decrement (up to p < 0.001) in plasma and urine urea + + and creatinine, urinary Na and K level, renal lipid peroxidation 
along with significant increment (up to p < 0.001) in renal enzymatic and non-enzymatic antioxidants. Histological observations of kidney tissues too correlated with the biochemical observations. These finding powerfully supports that Trichosanthes dioica leaves extract acts in the kidney as a potent scavenger of free radicals to prevent the toxic effects of GM both in the biochemical and histopathological parameters.